Modulación diferencial de la actividad enzimática lítica de la pared celular entre Trichoderma sp. y Bacillus subtilis durante el biocontrol de Colletotrichum gloeosporioides in vitro

Resumen

Colletotrichum gloeosporioides (agente causal de la antracnosis) genera pérdidas significativas en diversos cultivos. Es necesario desarrollar alternativas al control químico para combatir esta enfermedad. En este estudio, se evaluó la eficacia de los tratamientos simples o combinados de Trichoderma sp. y Bacillus subtilis sobre C. gloeosporioides en agar papa dextrosa (PDA) y Luria Bertani (LB). La actividad antagónica individual de Trichoderma sp. y B. subtilis fue mayor en LB. Se observó un efecto diferencial del grado de inhibición y de la actividad hidrolítica en algunas combinaciones de Trichoderma y Bacillus. Sin embargo, la combinación de Trichoderma T2 y B. subtilis mostró compatibilidad, ambas cepas mostraron actividad celulolítica y quitinolítica estable. Por otro lado, el micelio desactivado de C. gloeosporioides estimuló las actividades endo-β-1,3-glucanasa, endo-β-1,4-glucanasa y β-N-acetilhexosaminidasa de B. subtilis y de las cepas de Trichoderma (T2 y T3) excepto la β-N-acetilhexosaminidasa de Trichoderma T1. En el sistema tripartito la actividad hidrolítica disminuyó en la mayoría de los sistemas, aunque la actividad β-N-acetilhexosaminidasa incrementó notablemente. Estos resultados sugieren que la combinación de Trichoderma T2 y B. subtilis posee potencial antagónico sobre C. gloeosporioides independientemente del contenido nutricional, por lo que esta mezcla se podría emplear para el tratamiento de la antracnosis.

Palabras clave: agentes de control biológico, antracnosis, co-cultivo

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Publicado
2022-11-23
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Bautista-Ortega , P. I., Hernández-Hernández, I., Pérez-Pérez , R., Soria-Leal, S.-L. L. Y., & Chávez-Avilés, M. N. (2022). Modulación diferencial de la actividad enzimática lítica de la pared celular entre Trichoderma sp. y Bacillus subtilis durante el biocontrol de Colletotrichum gloeosporioides in vitro. Ciencia Latina Revista Científica Multidisciplinar, 6(6), 732-768. https://doi.org/10.37811/cl_rcm.v6i6.3568
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