Daño genotóxico en Macrobrachium tenellum (Smith, 1871) en canales de riego agrícola en Guasave, Sinaloa, México
Resumen
Macrobrachium tenellum es una especie económicamente importante, pero la contaminación natural y antropogénica pone en riesgo su presencia en la naturaleza. Los organismos se colectaron en canales de riego agrícola en Guasave, Sinaloa, México, se transportaron al laboratorio de Genotoxicología de la Universidad Autónoma de Occidente, Unidad Regional Los Mochis. Los organismos se sacrificaron por descenso de temperatura, se extrajo su hepatopáncreas, se maceró y una muestra se suspendió en 200 μL de solución fisiológica (PBS) a 37 °C, se tomó una alícuota para realizar el ensayo cometa. Se analizaron 36 organismos. El 69% presentó daño en ADN (72% hembras - 28% machos). Se observó una relación inversa entre talla y daño genotóxico. Se determinó que no existe relación entre el sexo y el daño genotóxico. El daño fue en igual proporción en hembras ovígeras y no ovígeras. M. tenellum tiene potencial para ser utilizado como biomonitor de sitios contaminados con mezclas de plaguicidas agrícolas.
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Derechos de autor 2023 Pedro Hernández-Sandoval , Abigail Lagarda-Escárrega , Jesús Roberto Alanis-Escalant, María del Carmen Martínez-Valenzuela, Jeovan Alberto Ávila-Díaz, David Váldez-Martínez, Arturo Arciniega-Galaviz
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