Efecto del cloruro de cadmio sobre la expresión del represor transcripcional REST/NRSF y su gen blanco CDH1 en tejido pulmonar de ratones ICR-CD1
Resumen
REST (RE1-Silencing Transcription factor) es un factor de transcripción con dedos de zinc que reprime a sus blancos transcripcionales a través de su interacción con la secuencia RE1 (Restrictive Element 1). Aunque algunos metales como el cadmio pueden alterar la función de proteínas al competir con el zinc, no se han realizado estudios a nivel molecular para para determinar dicho efecto sobre REST. El gen CDH1 es un blanco transcripcional de REST que codifica para la glicoproteína de adhesión celular E-cadherina y la desregulación de su expresión ha sido asociada al proceso canceroso. El objetivo de este trabajo fue evaluar el efecto de la exposición a 1.3 µM/3 días con CdCl2 en ratones ICR-CD1 sobre los niveles de REST y de su gen blanco CDH1 en tejido pulmonar. Los niveles del mRNA de CDH1 se determinaron por RT-PCR, mientras que los niveles de las proteínas E-cadherina y REST se evaluaron mediante Western blot. Después del tratamiento con CdCl2 los niveles de CDH1 y de su producto E-cadherina se incrementaron en relación con la pérdida de la expresión de REST. En conclusión, el cadmio promueve la disminución de REST a nivel de proteína, así como el incremento de los niveles de ARN mensajero de CDH1 y de su producto E-cadherina. El incremento de E-cadherina probablemente se debe a la relajación transcripcional de CDH1 mediado por la pérdida de la expresión de REST.
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Derechos de autor 2023 Francisco Yovani Vargas Santiago;Mayrut Osdely Urióstegui Acosta ;Aida Castillo;Yanet Romero Ramírez;Antonio Cuenca Rojo;Eduardo Castañeda Saucedo;Napoleón Navarro Tito;Miguel Ángel Mendoza Catalán;Carlos Ortuño Pineda
Esta obra está bajo licencia internacional Creative Commons Reconocimiento 4.0.
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