Análisis y Clonación del Gen catE de Bacillus Licheniformis M2-7
Resumen
En Bacillus licheniformis M2-7 el gen catE codifica para la posible proteína catecol 2, 3-dioxigenasa, relacionada con la biodegradación de hidrocarburos, confiriéndole mecanismos de resistencia contra estos compuestos. En los últimos años, el desarrollo de organismos genéticamente modificados (OGM) y la biorremediación utilizan esta nueva tecnología potenciando las características metabólicas y logrando una degradación de agentes contaminantes más eficiente. Para establecer estrategias de biorremediación, se debe conocer los procesos metabólicos implicados y los genes que codifican las proteínas relacionadas. Este trabajo se centra en analizar y clonar el gen catE de B. licheniformis M2-7 en un plásmido para confirmar su función en la utilización de hidrocarburos. Para ello se extrajo el ADN cromosomal, se amplificó un fragmento del gen catE mediante PCR y se clonó en el vector pJET1.2/blunt, la transformación se realizó E. coli DH5. La construcción del plásmido recombinante se confirmó mediante PCR y digestión con EcoRV. Mediante esta metodología se logró diseñar el plásmido pCAT derivado del vector pJET1.2/blunt, llevando un fragmento del gen catE de B. licheniformis M2-7, plásmido que se encuentra en la cepa RMJ de E. coli, lo que permitirá utilizarla como herramienta molecular para generar una mutante en dicho gen en B. licheniformis.
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Derechos de autor 2024 Rigoberto Mozo Mejía, Augusto Rojas Aparicio, Miguel Ángel Rodríguez Barrera, Carlos Ortuño Pineda, Yanet Romero Ramírez
Esta obra está bajo licencia internacional Creative Commons Reconocimiento 4.0.
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